Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing

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David Diouf

Abstract

Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity.

Public information

Title:

Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing

Authors:

David Diouf, Maria Rosaria Vitale, Johanna Eva Maria Zöller, Ana-Magdalena Pineau, Eva Klopocki, Catharina Hamann, Georg Christoph Ziegler, Tim Vanmierlo, Daniel Van den Hove, Klaus-Peter Lesch

Journal:

Stem Cell Research

Year:

2023

Url:

https://doi.org/10.1016/j.scr.2023.103038